Characterization of a nuclease produced by Staphylococcus aureus.
نویسندگان
چکیده
The conformational parameters of a nuclease produced by growing cultures of Staphylococcus aureus have been determined. This nuclease exhibits both ribonuclease and deoxyribonuclease activities. It has been shown to have a pH optimum of 9.2 and an absolute requirement for calcium ions. The experimental data are compatible with a compact globular protein of mol wt 17,000 and with a helical content of approximately 45 % .
منابع مشابه
The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. II. The amino acid sequences of tryptic and chymotryptic peptides.
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متن کاملComparison of coagulase , deoxyribonuclease ( DNase ) , and heat - stable nuclease tests for identification of Staphylococcus aureus ROSALIE
One thousand and thirty-five clinical isolates of the genus staphylococcus were used to compare the coagulase test with the deoxyribonuclease (DNase) and heat-stable nuclease tests as methods for identifying Staphylococcus aureus. Conflicting results were obtained with 65 isolates when the coagulase test was compared with the DNase test but with only one isolate when the coagulase test was comp...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 242 5 شماره
صفحات -
تاریخ انتشار 1967